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gramd4 antibody clone c 8  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology gramd4 antibody clone c 8
    (A) Manhattan plot showing all splice variant SNPs across the 49 phenotypes. For each splice variant, the best p value observed among all assessed traits is plotted on a –log10 scale (y axis), according to its genomic coordinates (x axis). <t>Gramd4</t> splice variant chosen for validation is highlighted in red. (B) Variation in frequency of BM pre-cDCs in C57BL/6J (gray; n = 3), 129 (pink; n = 3), NOD (blue; n = 3), NZO (cyan; n = 3), A/J (yellow; n = 3), WSB (purple; n = 3), DO (black; n = 170), and CC-RI (open; n = 47) mice. (C) A QTL driving the frequency of BM pre-cDCs found within chromosome 15 (chr15:86,581,607, LOD = 10.7) that appears to be driven by an A/J and NZO founder effect. (D) Gramd4 gene structure and schematic representation of alternative splicing (UCSC Genome Browser). SNP localization is shown in red. (E) Western blot on total splenocytes from Gramd4 w/w and Gramd4 sp/sp mice showing alternative splicing. (F) Schematic representation of mixed BM chimera experiment. (G) Representative flow cytometry plot for mixed BM experiment. (H) Frequencies of DC progenitors and subsets in mixed BM chimera mice due to differential expression of Gramd4 SNP variant in BM, spleen, and inguinal LN. Chimerism is expressed as the ratio between the number of CD45.2 and CD45.1/CD45.2 cells for each cell population. Representative of 2 independent experiments; each dot represents one mouse, n = 6 per group, Gramd4 w/w (red) and Gramd4 sp/sp (blue), and horizontal lines represent means. (I) Schematic representation of the experimental setup in (J) and (K). (J) Representative flow cytometry plot for OT-II CD4 + T cell activation (CTV dilution after immunization with OVA 328–339 peptide in alum) in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Graph shows the absolute numbers of OT-II cells in popliteal lymph nodes and the x axis the number of divisions after immunization. (K) CD69 and CD43 expression of divided OT-II T cells in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Each dot represents one mouse, n = 6 per group, Gramd4 w/w (orange) and Gramd4 sp/sp (green), and horizontal lines represent means (J and K). Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. iLN (inguinal LN).
    Gramd4 Antibody Clone C 8, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gramd4 antibody clone c 8/product/Santa Cruz Biotechnology
    Average 88 stars, based on 3 article reviews
    gramd4 antibody clone c 8 - by Bioz Stars, 2026-03
    88/100 stars

    Images

    1) Product Images from "Quantitative trait loci mapping provides insights into the genetic regulation of dendritic cell numbers in mouse tissues"

    Article Title: Quantitative trait loci mapping provides insights into the genetic regulation of dendritic cell numbers in mouse tissues

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.114296

    (A) Manhattan plot showing all splice variant SNPs across the 49 phenotypes. For each splice variant, the best p value observed among all assessed traits is plotted on a –log10 scale (y axis), according to its genomic coordinates (x axis). Gramd4 splice variant chosen for validation is highlighted in red. (B) Variation in frequency of BM pre-cDCs in C57BL/6J (gray; n = 3), 129 (pink; n = 3), NOD (blue; n = 3), NZO (cyan; n = 3), A/J (yellow; n = 3), WSB (purple; n = 3), DO (black; n = 170), and CC-RI (open; n = 47) mice. (C) A QTL driving the frequency of BM pre-cDCs found within chromosome 15 (chr15:86,581,607, LOD = 10.7) that appears to be driven by an A/J and NZO founder effect. (D) Gramd4 gene structure and schematic representation of alternative splicing (UCSC Genome Browser). SNP localization is shown in red. (E) Western blot on total splenocytes from Gramd4 w/w and Gramd4 sp/sp mice showing alternative splicing. (F) Schematic representation of mixed BM chimera experiment. (G) Representative flow cytometry plot for mixed BM experiment. (H) Frequencies of DC progenitors and subsets in mixed BM chimera mice due to differential expression of Gramd4 SNP variant in BM, spleen, and inguinal LN. Chimerism is expressed as the ratio between the number of CD45.2 and CD45.1/CD45.2 cells for each cell population. Representative of 2 independent experiments; each dot represents one mouse, n = 6 per group, Gramd4 w/w (red) and Gramd4 sp/sp (blue), and horizontal lines represent means. (I) Schematic representation of the experimental setup in (J) and (K). (J) Representative flow cytometry plot for OT-II CD4 + T cell activation (CTV dilution after immunization with OVA 328–339 peptide in alum) in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Graph shows the absolute numbers of OT-II cells in popliteal lymph nodes and the x axis the number of divisions after immunization. (K) CD69 and CD43 expression of divided OT-II T cells in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Each dot represents one mouse, n = 6 per group, Gramd4 w/w (orange) and Gramd4 sp/sp (green), and horizontal lines represent means (J and K). Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. iLN (inguinal LN).
    Figure Legend Snippet: (A) Manhattan plot showing all splice variant SNPs across the 49 phenotypes. For each splice variant, the best p value observed among all assessed traits is plotted on a –log10 scale (y axis), according to its genomic coordinates (x axis). Gramd4 splice variant chosen for validation is highlighted in red. (B) Variation in frequency of BM pre-cDCs in C57BL/6J (gray; n = 3), 129 (pink; n = 3), NOD (blue; n = 3), NZO (cyan; n = 3), A/J (yellow; n = 3), WSB (purple; n = 3), DO (black; n = 170), and CC-RI (open; n = 47) mice. (C) A QTL driving the frequency of BM pre-cDCs found within chromosome 15 (chr15:86,581,607, LOD = 10.7) that appears to be driven by an A/J and NZO founder effect. (D) Gramd4 gene structure and schematic representation of alternative splicing (UCSC Genome Browser). SNP localization is shown in red. (E) Western blot on total splenocytes from Gramd4 w/w and Gramd4 sp/sp mice showing alternative splicing. (F) Schematic representation of mixed BM chimera experiment. (G) Representative flow cytometry plot for mixed BM experiment. (H) Frequencies of DC progenitors and subsets in mixed BM chimera mice due to differential expression of Gramd4 SNP variant in BM, spleen, and inguinal LN. Chimerism is expressed as the ratio between the number of CD45.2 and CD45.1/CD45.2 cells for each cell population. Representative of 2 independent experiments; each dot represents one mouse, n = 6 per group, Gramd4 w/w (red) and Gramd4 sp/sp (blue), and horizontal lines represent means. (I) Schematic representation of the experimental setup in (J) and (K). (J) Representative flow cytometry plot for OT-II CD4 + T cell activation (CTV dilution after immunization with OVA 328–339 peptide in alum) in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Graph shows the absolute numbers of OT-II cells in popliteal lymph nodes and the x axis the number of divisions after immunization. (K) CD69 and CD43 expression of divided OT-II T cells in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Each dot represents one mouse, n = 6 per group, Gramd4 w/w (orange) and Gramd4 sp/sp (green), and horizontal lines represent means (J and K). Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. iLN (inguinal LN).

    Techniques Used: Variant Assay, Biomarker Discovery, Alternative Splicing, Western Blot, Flow Cytometry, Quantitative Proteomics, Activation Assay, Expressing

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Marker, Staining, Recombinant, Red Blood Cell Lysis, Software



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    gramd4 antibody clone c 8  (Santa Cruz Biotechnology)
    88
    Santa Cruz Biotechnology gramd4 antibody clone c 8
    (A) Manhattan plot showing all splice variant SNPs across the 49 phenotypes. For each splice variant, the best p value observed among all assessed traits is plotted on a –log10 scale (y axis), according to its genomic coordinates (x axis). <t>Gramd4</t> splice variant chosen for validation is highlighted in red. (B) Variation in frequency of BM pre-cDCs in C57BL/6J (gray; n = 3), 129 (pink; n = 3), NOD (blue; n = 3), NZO (cyan; n = 3), A/J (yellow; n = 3), WSB (purple; n = 3), DO (black; n = 170), and CC-RI (open; n = 47) mice. (C) A QTL driving the frequency of BM pre-cDCs found within chromosome 15 (chr15:86,581,607, LOD = 10.7) that appears to be driven by an A/J and NZO founder effect. (D) Gramd4 gene structure and schematic representation of alternative splicing (UCSC Genome Browser). SNP localization is shown in red. (E) Western blot on total splenocytes from Gramd4 w/w and Gramd4 sp/sp mice showing alternative splicing. (F) Schematic representation of mixed BM chimera experiment. (G) Representative flow cytometry plot for mixed BM experiment. (H) Frequencies of DC progenitors and subsets in mixed BM chimera mice due to differential expression of Gramd4 SNP variant in BM, spleen, and inguinal LN. Chimerism is expressed as the ratio between the number of CD45.2 and CD45.1/CD45.2 cells for each cell population. Representative of 2 independent experiments; each dot represents one mouse, n = 6 per group, Gramd4 w/w (red) and Gramd4 sp/sp (blue), and horizontal lines represent means. (I) Schematic representation of the experimental setup in (J) and (K). (J) Representative flow cytometry plot for OT-II CD4 + T cell activation (CTV dilution after immunization with OVA 328–339 peptide in alum) in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Graph shows the absolute numbers of OT-II cells in popliteal lymph nodes and the x axis the number of divisions after immunization. (K) CD69 and CD43 expression of divided OT-II T cells in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Each dot represents one mouse, n = 6 per group, Gramd4 w/w (orange) and Gramd4 sp/sp (green), and horizontal lines represent means (J and K). Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. iLN (inguinal LN).
    Gramd4 Antibody Clone C 8, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gramd4 antibody clone c 8/product/Santa Cruz Biotechnology
    Average 88 stars, based on 1 article reviews
    gramd4 antibody clone c 8 - by Bioz Stars, 2026-03
    88/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Manhattan plot showing all splice variant SNPs across the 49 phenotypes. For each splice variant, the best p value observed among all assessed traits is plotted on a –log10 scale (y axis), according to its genomic coordinates (x axis). Gramd4 splice variant chosen for validation is highlighted in red. (B) Variation in frequency of BM pre-cDCs in C57BL/6J (gray; n = 3), 129 (pink; n = 3), NOD (blue; n = 3), NZO (cyan; n = 3), A/J (yellow; n = 3), WSB (purple; n = 3), DO (black; n = 170), and CC-RI (open; n = 47) mice. (C) A QTL driving the frequency of BM pre-cDCs found within chromosome 15 (chr15:86,581,607, LOD = 10.7) that appears to be driven by an A/J and NZO founder effect. (D) Gramd4 gene structure and schematic representation of alternative splicing (UCSC Genome Browser). SNP localization is shown in red. (E) Western blot on total splenocytes from Gramd4 w/w and Gramd4 sp/sp mice showing alternative splicing. (F) Schematic representation of mixed BM chimera experiment. (G) Representative flow cytometry plot for mixed BM experiment. (H) Frequencies of DC progenitors and subsets in mixed BM chimera mice due to differential expression of Gramd4 SNP variant in BM, spleen, and inguinal LN. Chimerism is expressed as the ratio between the number of CD45.2 and CD45.1/CD45.2 cells for each cell population. Representative of 2 independent experiments; each dot represents one mouse, n = 6 per group, Gramd4 w/w (red) and Gramd4 sp/sp (blue), and horizontal lines represent means. (I) Schematic representation of the experimental setup in (J) and (K). (J) Representative flow cytometry plot for OT-II CD4 + T cell activation (CTV dilution after immunization with OVA 328–339 peptide in alum) in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Graph shows the absolute numbers of OT-II cells in popliteal lymph nodes and the x axis the number of divisions after immunization. (K) CD69 and CD43 expression of divided OT-II T cells in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Each dot represents one mouse, n = 6 per group, Gramd4 w/w (orange) and Gramd4 sp/sp (green), and horizontal lines represent means (J and K). Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. iLN (inguinal LN).

    Journal: Cell reports

    Article Title: Quantitative trait loci mapping provides insights into the genetic regulation of dendritic cell numbers in mouse tissues

    doi: 10.1016/j.celrep.2024.114296

    Figure Lengend Snippet: (A) Manhattan plot showing all splice variant SNPs across the 49 phenotypes. For each splice variant, the best p value observed among all assessed traits is plotted on a –log10 scale (y axis), according to its genomic coordinates (x axis). Gramd4 splice variant chosen for validation is highlighted in red. (B) Variation in frequency of BM pre-cDCs in C57BL/6J (gray; n = 3), 129 (pink; n = 3), NOD (blue; n = 3), NZO (cyan; n = 3), A/J (yellow; n = 3), WSB (purple; n = 3), DO (black; n = 170), and CC-RI (open; n = 47) mice. (C) A QTL driving the frequency of BM pre-cDCs found within chromosome 15 (chr15:86,581,607, LOD = 10.7) that appears to be driven by an A/J and NZO founder effect. (D) Gramd4 gene structure and schematic representation of alternative splicing (UCSC Genome Browser). SNP localization is shown in red. (E) Western blot on total splenocytes from Gramd4 w/w and Gramd4 sp/sp mice showing alternative splicing. (F) Schematic representation of mixed BM chimera experiment. (G) Representative flow cytometry plot for mixed BM experiment. (H) Frequencies of DC progenitors and subsets in mixed BM chimera mice due to differential expression of Gramd4 SNP variant in BM, spleen, and inguinal LN. Chimerism is expressed as the ratio between the number of CD45.2 and CD45.1/CD45.2 cells for each cell population. Representative of 2 independent experiments; each dot represents one mouse, n = 6 per group, Gramd4 w/w (red) and Gramd4 sp/sp (blue), and horizontal lines represent means. (I) Schematic representation of the experimental setup in (J) and (K). (J) Representative flow cytometry plot for OT-II CD4 + T cell activation (CTV dilution after immunization with OVA 328–339 peptide in alum) in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Graph shows the absolute numbers of OT-II cells in popliteal lymph nodes and the x axis the number of divisions after immunization. (K) CD69 and CD43 expression of divided OT-II T cells in popliteal LN of Gramd4 w/w and Gramd4 sp/sp recipient mice. Each dot represents one mouse, n = 6 per group, Gramd4 w/w (orange) and Gramd4 sp/sp (green), and horizontal lines represent means (J and K). Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. iLN (inguinal LN).

    Article Snippet: Gramd4 antibody _ Clone C-8 , Santa-Cruz , Cat No. sc-515128.

    Techniques: Variant Assay, Biomarker Discovery, Alternative Splicing, Western Blot, Flow Cytometry, Quantitative Proteomics, Activation Assay, Expressing

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Quantitative trait loci mapping provides insights into the genetic regulation of dendritic cell numbers in mouse tissues

    doi: 10.1016/j.celrep.2024.114296

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Gramd4 antibody _ Clone C-8 , Santa-Cruz , Cat No. sc-515128.

    Techniques: Marker, Staining, Recombinant, Red Blood Cell Lysis, Software